Regulatory

Part:BBa_K305006:Design

Designed by: Joel Kuiper, Arend Jan Suk   Group: iGEM10_Groningen   (2010-09-30)

yqxM promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 280
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 250


Design Notes

The sequence was retrieved from genomic Bacillus subtilis DNA using PCR with the following primers:

yqxM-for: CTGACTGCAGCGGCCGCTACTAGTACTGTAACTTGATATGACAATCG

yqxM-rev: ATCAGAATTCGCGGCCGCTTCTAGAGAGCGGTGTTTCTTCTGCGTG


Source

[http://dbtbs.hgc.jp/COG/prom/yqxM-sipW-tasA.html dbtbs]

References